Name of instrument, m RealTime System composed of the msp and mrt modules. Country where designed/Manufactured/Reagents. The Abbott m System comprises the msp and mrz instruments, which are operated with separate System Control Center (SCC). J Clin Microbiol. Jun;47(6) doi: /JCM Epub Apr Performance of the Abbott real-time PCR assay using msp and .
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Table 1 summarizes the assay characteristics of these two assays.
This method has now become a standard method for monitoring HIV-infected patients on antiretroviral therapy in the United States. Select your language of interest to view the total content in your interested language. The Abbott method positively identified samples out of with the detectability of An internal control was added into the samples preparation procedure.
Our data showed that these two methods are essentially indistinguishable with a correlation coefficient of 0.
Even though both the Roche and Abbott methods use the realtime PCR technology, the gene targets are different. HIV viral particles were first lysed and an HIV-1 Quantitation Standard QS Armored RNA molecule was added to each sample, which was followed by a series of processes which involves incubation, introduction of magnetic particles, washing to remove unbound particles and elution of nucleic acid NA at elevated temperatures.
The extracted NA was mixed with the amplification reagents before amplification with the mrt Abbott, a ; Abbott, m0200sp Of ND Roche samples, 15 of them Receive your quote m20000sp from the manufacturer.
m2000 REALTIME SYSTEM
Automation is the foundation for the clinical molecular laboratory to report patient results m200s0p confidence. The fluorescence counts were converted into VL measure by the analyzer Abbott, a ; Abbott, A total of paired abhott samples were tested in the same day by both methods Table 2. Together, these data suggest that neither method is better than the other for measuring VL. The Abbott method uses real-time platforms with fluorescence detection systems Abbott, b ; Scott et al.
All blood samples were collected in EDTA tubes. Real-time PCR was used for detection.
Another common feature of these two assays is the use of realtime PCR as their underlying technology for the measurement of HIV- 1 viral load. Save time Submit your details once and make multiple inquiries.
The Abbott mrt performed the automated amplification and detection process.
However, a relative significant percentage This study was approved by the Institutional Research Board under the protocol number of H Be the first to review this product. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
This automated system uses magnetic microparticles-based reagents for the purification of nucleic acid from the samples. One way to further evaluate the cause of the observed discrepancies is to determine the HIV-1 subtypes by DNA sequencing. Different from k2000sp conventional PCR, an internal probe is added to the detection process, which is an oligo nucleotide with both a fluorescent reporter and a m200sp quencher dye attached.
The solid line abbitt the box indicates the median value and the ends of the whiskers indicate the minimum and maximum values.
Future work includes increase of the testing population size, determination of the HIV-1 subtypes and DNA sequence analysis of those discrepant samples. The vertical axis represenst the VL differences the Abbott method minus the Roche method and the horizontal axis represenst the means of VL.
The results showed as.
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The number of samples within this range was It runs 96 samples per run per day with more if done overnight. Results of this study are unable to evaluate the potential differences between these two assays in this regard. This automation enables laboratorians to interface where their skills best contribu October 18, Citation: Other assay performance characteristics such as analytical specificity, linearity, reproducibility, and precision were not evaluated.
Out of paired plasma tested, Short Communication Open Access.